Vinculin Protein (VCL) (AA 2-1066)

Catalog No. ABIN7313964

This Recombinant Vinculin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Vinculin Protein (VCL) (AA 2-1066) (ABIN7313964)

Target: Vinculin (VCL)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-1066

Purpose: Recombinant Human VCL Protein is produced by our E. coli expression system and the target gene encoding Pro2-Gln1066 is expressed.

Sequence: PVFHTRTIES ILEPVAQQIS HLVIMHEEGE VDGKAIPDLT APVAAVQAAV SNLVRVGKET VQTTEDQILK RDMPPAFIKV ENACTKLVQA AQMLQSDPYS VPARDYLIDG SRGILSGTSD LLLTFDEAEV RKIIRVCKGI LEYLTVAEVV ETMEDLVTYT KNLGPGMTKM AKMIDERQQE LTHQEHRVML VNSMNTVKEL LPVLISAMKI FVTTKNSKNQ GIEEALKNRN FTLEKMSAEI NEIIRVLQLT SWDEDAWASK DTEAMKRALA SIDSKLNQAK GWLRDPSASP GDAGEQAIRQ ILDEAGKVGE LCAGKERREI LGTCKMLGQM TDQVADLRAR GQGSSPVAMQ KAQQVSQGLD VLTAKVENAA RKLEAMTNSK QSIAKKIDAA QNWLADPNGG PEGEEQIRGA LAEARKIAEL CDDPKERDDI LRSLGEISAL TSKLADLRRQ GKGDSPEARA LAKQVATALQ NLQTKTNRAV ANSRPAKAAV HLEGKIEQAQ RWIDNPTVDD RGVGQAAIRG LVAEGHRLAN VMMGPYRQDL LAKCDRVDQL TAQLADLAAR GEGESPQARA LASQLQDSLK DLKARMQEAM TQEVSDVFSD TTTPIKLLAV AATAPPDAPN REEVFDERAA NFENHSGKLG ATAEKAAAVG TANKSTVEGI QASVKTAREL TPQVVSAARI LLRNPGNQAA YEHFETMKNQ WIDNVEKMTG LVDEAIDTKS LLDASEEAIK KDLDKCKVAM ANIQPQMLVA GATSIARRAN RILLVAKREV ENSEDPKFRE AVKAASDELS KTISPMVMDA KAVAGNISDP GLQKSFLDSG YRILGAVAKV REAFQPQEPD FPPPPPDLEQ LRLTDELAPP KPPLPEGEVP PPRPPPPEEK DEEFPEQKAG EVINQPMMMA ARQLHDEARK WSSKGNDIIA AAKRMALLMA EMSRLVRGGS GTKRALIQCA KDIAKASDEV TRLAKEVAKQ CTDKRIRTNL LQVCERIPTI STQLKILSTV KATMLGRTNI SDEESEQATE MLVHNAQNLM QSVKETVREA EAASIKIRTD AGFTLRWVRK TPWYQ

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: Vinculin (VCL)

Alternative Name: VCL

Gene ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2