Nerve Growth Factor Protein (NGF) (AA 130-239)

Catalog No. ABIN7313026

This Recombinant Nerve Growth Factor protein is produced in Escherichia coli (E. coli).

Quick Overview for Nerve Growth Factor Protein (NGF) (AA 130-239) (ABIN7313026)

Target: Nerve Growth Factor (NGF)

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 130-239

Purpose: Recombinant Mouse NGF Protein (Met130-Arg239) is produced by our E. coli expression system and the target gene encoding Met130-Arg239 is expressed.

Sequence: MGEFSVCDSV SVWVGDKTTA TDIKGKEVTV LAEVNINNSV FRQYFFETKC RASNPVESGC RGIDSKHWNS YCTTTHTFVK ALTTDEKQAA WRFIRIDTAC VCVLSRKATR

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM Tris, 200 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Pathways: Regulation of Cell Size