Histone H3.1 Protein (His tag)

Catalog No. ABIN5505656

Recombinant Histone H3.1 protein expressed in Escherichia coli (E. coli).

Quick Overview for Histone H3.1 Protein (His tag) (ABIN5505656)

Target: Histone H3.1 (HIST1H3B) (Histone Cluster 1, H3b (HIST1H3B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Histone H3.1 protein is labelled with His tag.

Characteristics:

• Recombinant human Purified recombinant protein of Human histone cluster 1, H3b (HIST1H3B), full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Purification: Purified

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Histone H3.1 (HIST1H3B) (Histone Cluster 1, H3b (HIST1H3B))

Alternative Name: histone cluster 1, H3b

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Molecular Weight: 15.2 kDa

NCBI Accession: NP_003528