PET100 Protein (His tag)

Catalog No. ABIN5505663

This Recombinant PET100 protein is produced in Escherichia coli (E. coli).

Quick Overview for PET100 Protein (His tag) (ABIN5505663)

Target: PET100 (PET100 Homolog (PET100))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This PET100 protein is labelled with His tag.

Characteristics:

• Recombinant human Purified recombinant protein of Human hypothetical protein LOC100131801 (LOC100131801), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.coli, 50 μg (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Purification: Purified

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PET100 (PET100 Homolog (PET100))

Alternative Name: LOC100131801

Background: Mitochondrial complex IV, or cytochrome c oxidase, is a large transmembrane protein complex that is part of the respiratory electron transport chain of mitochondria. The small protein encoded by this gene plays a role in the biogenesis of mitochondrial complex IV. This protein localizes to the inner mitochondrial membrane and is exposed to the intermembrane space. Mutations in this gene are associated with mitochondrial complex IV deficiency. This gene has a pseudogene on chromosome 3. Alternative splicing results in multiple transcript variants.

Molecular Weight: 9.6 kDa

NCBI Accession: NP_001164626