Quick Overview for IL23A Protein (ABIN5505700)
Target
See all IL23A ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Interleukin-23 alpha / IL23A protein expressed in Insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 0.5 - 0.8 ng/ml, corresponding to a specific activity of 1.25-2.0 x 10^6 units/mg, determined by mouse splenocytes IL-17A secretion induced by mIL-23 in a dose dependent manner. Under this assay, the bioactivity is equivalent
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Interleukin 23, alpha subunit p19 (IL23A) (AA 1-189) protein (GST tag)
IL23A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 23, alpha subunit p19 (IL23A) (AA 20-189) protein (Fc Tag)IL23A Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Interleukin 23, alpha subunit p19 (IL23A) (AA 22-196) protein (Fc Tag)IL23A Origin: Mouse Host: Mammalian Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 23, alpha subunit p19 (IL23A) (AA 20-157) protein (His-IF2DI Tag)IL23A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 23, alpha subunit p19 (IL23A) (AA 22-196) (Active) protein (Biotin,His-Avi Tag)IL23A Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 1 x PBS
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL23A (Interleukin 23, alpha subunit p19 (IL23A))
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Alternative Name
- Interleukin-23 alpha / IL23A
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Molecular Weight
- 55.4 kDa
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NCBI Accession
- NP_112542
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Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Target
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