RASGRP3 Protein (GST tag,His tag)
Quick Overview for RASGRP3 Protein (GST tag,His tag) (ABIN5505756)
Target
See all RASGRP3 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This RASGRP3 protein is labelled with GST tag,His tag.
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Characteristics
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- Recombinant human Purified recombinant protein of Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50 μg (full length, N-term GST tag, C-term His tag, transcript variant 2) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
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Purification
- Purified
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RAS Guanyl Releasing Protein 3 (Calcium and DAG-Regulated) (RASGRP3) (AA 1-690) protein (GST tag)
RASGRP3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
RAS Guanyl Releasing Protein 3 (Calcium and DAG-Regulated) (RASGRP3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)RASGRP3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal,N-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- RASGRP3 (RAS Guanyl Releasing Protein 3 (Calcium and DAG-Regulated) (RASGRP3))
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Alternative Name
- RAS guanyl releasing protein 3
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Background
- Members of the RAS (see HRAS MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008].
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Molecular Weight
- 104.2 kDa
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NCBI Accession
- NP_733772
Target
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