RPL28 Protein (GST tag,His tag)

Catalog No. ABIN5505763

This Recombinant RPL28 protein is expressed in Escherichia coli (E. coli).

Quick Overview for RPL28 Protein (GST tag,His tag) (ABIN5505763)

Target: RPL28 (Ribosomal Protein L28 (RPL28))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This RPL28 protein is labelled with GST tag,His tag.

Characteristics:

• Recombinant human Purified recombinant protein of Human ribosomal protein L28 (RPL28), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50 μg (full length, N-term GST tag, C-term His tag, transcript variant 2) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Purification: Purified

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal,N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RPL28 (Ribosomal Protein L28 (RPL28))

Alternative Name: ribosomal protein L28

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008].

Molecular Weight: 41.6 kDa

NCBI Accession: NP_000982