Clusterin Protein (CLU) (AA 1-449, Secretory Component) (DYKDDDDK Tag)

Catalog No. ABIN5564496

This Recombinant Clusterin protein is produced in HEK-293 Cells.

Quick Overview for Clusterin Protein (CLU) (AA 1-449, Secretory Component) (DYKDDDDK Tag) (ABIN5564496)

Target: Clusterin (CLU)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 1-449, Secretory Component

Purification tag / Conjugate: This Clusterin protein is labelled with DYKDDDDK Tag.

Purpose: Clusterin (secretory form) (human) (rec.)

Cross-Reactivity: Human

Characteristics: Signal peptide sequence and the human secretory clusterin (aa 1-449) are fused at the C-terminus to a FLAG®-tag.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: Clusterin (CLU)

Alternative Name: Clusterin

Background:

TRPM-2, Apolipoprotein J, APO-J, CLI, CLU, SGP-2

Clusterin shares homology with the small heat shock protein family of molecular chaperones. The mature secreted form of the protein is a glycosylated, 80 kDa disulfide-linked heterodimer of alpha and beta subunits (produced by internal cleavage). Clusterin is expressed in virtually all tissues and found in all human fluids. It is involved in numerous physiological processes important for carcinogenesis and tumor growth, including apoptotic cell death, cell cycle regulation, DNA repair, cell adhesion, tissue remodeling, lipid transportation, membrane recycling and immune system regulation. Clusterin also exists as a nuclear protein. The secreted form of Clusterin has extracellular chaperone and anti-apoptotic activities while the nuclear form acts as a proapoptotic factor.

Molecular Weight: 80kDa (under non-reducing conditions), 39kDa and 40kDa (under reducing conditions) (by SDS-PAGE).

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling