Carbonic Anhydrase Protein (CA) (AA 1-220) (His tag)

Catalog No. ABIN5623634

Recombinant Carbonic Anhydrase protein expressed in Escherichia coli (E. coli).

Quick Overview for Carbonic Anhydrase Protein (CA) (AA 1-220) (His tag) (ABIN5623634)

Target: Carbonic Anhydrase (CA)

Protein Type: Recombinant

Biological Activity: Active

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-220

Purification tag / Conjugate: This Carbonic Anhydrase protein is labelled with His tag.

Sequence: 1-220 aa: MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK

Characteristics: Purified Recombinant Carbonic anhydrase protein (His tagged)

Purification: Purified

Biological Activity Comment: Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied in liquid form in 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Carbonic Anhydrase (CA)

Alternative Name: Carbonic anhydrase