IdhA (AA 1-329) (Active) protein (His tag)

Catalog No. ABIN5623662

This Recombinant IdhA protein is produced in Escherichia coli (E. coli).

Quick Overview for IdhA (AA 1-329) (Active) protein (His tag) (ABIN5623662)

Target: IdhA

Protein Type: Recombinant

Biological Activity: Active

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-329

Purification tag / Conjugate: His tag

Sequence: 1-329 aa: MGSSHHHHHH SSGLVPRGSH MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL

Characteristics: Purified Recombinant IdhA protein (His tagged)

Purification: Purified

Biological Activity Comment: Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied in liquid form in 20 mM Tris-HCl buffer, pH 8.0, 10 % glycerol, 100 mM NaCl

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IdhA