Surface Lipoprotein p27 Protein
Origin: Borrelia burgdorferi
Host: Escherichia coli (E. coli)
Recombinant
ELISA, WB
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- Target
- Surface Lipoprotein p27
- Protein Type
- Recombinant
- Origin
- Borrelia burgdorferi
- Source
- Escherichia coli (E. coli)
- Application
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 000-001-c30
- Supplier
- Rockland
- Purification
- Surface Lipoprotein p27 is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Surface Lipoprotein p27 and was estimated to be greater than 95% pure.
- Sterility
- Sterile filtered
-
- Application Notes
-
Application Note: Surface Lipoprotein p27 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 73.3 kDa for p27-MBP, (30.9 kDa for p27 and 42.4 kDa for MBP) in size corresponding to Surface Lipoprotein p27 by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 6 months
-
- Target
- Surface Lipoprotein p27
- Gene ID
- 1194336
- UniProt
- O50951
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