Surface Lipoprotein p27 Protein
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- Target
- Surface Lipoprotein p27
- Protein Type
- Recombinant
- Origin
- Borrelia burgdorferi
- Source
- Escherichia coli (E. coli)
- Application
- Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Supplier Product No.
- 000-001-c30
- Supplier
- Rockland
- Purpose
- Surface Lipoprotein p27 Control Protein
- Purification
- Surface Lipoprotein p27 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Surface Lipoprotein p27 and was estimated to be greater than 90% pure.
- Sterility
- Sterile filtered
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- Application Notes
-
Application Note: Surface Lipoprotein p27 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 73.3 kDa for p27-MBP, (30.9 kDa for p27 and 42.4 kDa for MBP) in size corresponding to Surface Lipoprotein p27 by Western blotting in the appropriate cell lysate or extract. Surface Lipoprotein p27 protein was tested in SDS-page and western blot.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: Lateral Flow Assay: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 6 months
-
- Target
- Surface Lipoprotein p27
- Background
-
Synonyms: BBA060 protein, Borrelia burgdorferi p27, Surface lipoprotein p27, control protein
Background: Surface Lipoprotein p27 of Borrelia burgdorferi is a surface-exposed lipoprotein that has been shown (by Western blot and Northern blot) to be expressed in the European B. burgdorferi strain B29, but not in the American strain B31. Cell envelope proteins of bacterial pathogens play important roles in the host-parasite interactions that occur during infection, including cell adherence, cell invasion, and immune cell activation or evasion. p27 is a basic protein of 248 amino acids with a typical prokaryotic leader sequence of 17 amino acid residues at the N-terminus of the proposed translation product. The p27 gene is located on a linear plasmid of a size of approximately 55 kb. Borrelia spirochetes are unique among diderm bacteria in their abundance of surface-displayed lipoproteins, some of which play important roles in the pathogenesis of Lyme disease and relapsing fever. There is evidence that Borrelia lipoproteins are specifically targeted to the bacterial surface, but that they can be retained in the periplasm by sequence-specific signals. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.
- Gene ID
- 1194336
- NCBI Accession
- WP_010890395
- UniProt
- O50951
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