ErpD/Arp37 Protein

Catalog No. ABIN5624581

Supplier Product No.: 000-001-c09

This Recombinant ErpD/Arp37 protein is produced in Escherichia coli (E. coli).

Quick Overview for ErpD/Arp37 Protein (ABIN5624581)

Target: ErpD/Arp37

Protein Type: Recombinant

Origin: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Product Details

Supplier Product No.: 000-001-c09

Supplier: Rockland

Purpose: Erpd/Arp37 Control Protein

Purification: ErpD/Arp37 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified ErpD/Arp37 and was estimated to be greater than 90% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: ErpD/Arp37 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 78 kDa for Arp37-MBP, (~35.6 kDa for Arp37 and 42.4 kDa for MBP) in size corresponding to ErpD/Arp37 by Western blotting in the appropriate cell lysate or extract. ErpD/Arp37 Protein has been tested in SDS-page and western blot.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: ErpD/Arp37

Background:

Synonyms: Arp37, Borrelia burgdorferi ErpD, control protein

Background: ErpD (ospE/F-Related Protein D), also known as Arp37, is from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. Erp proteins from Borrelia burgdorferi are postulated to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, indicative of Erp synthesis during the initial stages of vertebrate infection. It is postulated that surface-exposed Erp proteins could facilitate interactions with host tissues during the establishment of vertebrate infection. Lyme disease proteins are ideal for researchers interested in immunology, neurology, and rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

Gene ID: 1194073

NCBI Accession: WP_010890266

UniProt: G5IXI1