YBX3/DBPA Protein
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- Target See all YBX3/DBPA (YBX3) products
- YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))
- Protein Type
- Recombinant
- Origin
- Borrelia burgdorferi
- Source
- Escherichia coli (E. coli)
- Application
- Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Supplier Product No.
- 000-001-b98
- Supplier
- Rockland
- Purpose
- DbpA Control Protein
- Purification
- DbpA is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Decorin Binding Protein A and was estimated to be greater than 90% pure.
- Sterility
- Sterile filtered
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- Application Notes
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Application Note: DbpA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 60.9 kDa for DbpA-MBP, (18.5 kDa for DbpA and 42.4 kDa for MBP) in size corresponding to DbpA by Western blotting in the appropriate cell lysate or extract. Decorin Binding Protein A has been tested in SDS-page and western blot.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: Lateral Flow Assay: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 6 months
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- Target
- YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))
- Alternative Name
- DbpA (YBX3 Products)
- Background
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Synonyms: Decorin-binding Protein A, Borrelia burgdorferi dbpA, control protein, DbpA Control Protein
Background: DbpA, or Decorin Binding Protein A is from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. DbpA from other microbial organisms such as E. coli (ATP-dependent RNA helicase DbpA) are significantly different. The spirochete migrates from the tick midgut during tick feeding to tick salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize in mammalian tissues. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.
- Gene ID
- 1194347
- NCBI Accession
- WP_010890380
- UniProt
- O50917
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