CRASP-1 Protein

Catalog No. ABIN5624584

Supplier Product No.: 000-001-c18

This Recombinant CRASP-1 protein is produced in Escherichia coli (E. coli).

Quick Overview for CRASP-1 Protein (ABIN5624584)

Target: CRASP-1

Protein Type: Recombinant

Origin: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Product Details

Supplier Product No.: 000-001-c18

Supplier: Rockland

Purpose: Crasp-1 Control Protein

Purification: Crasp1 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Crasp1 and was estimated to be greater than 90% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: Crasp1 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 69.3 kDa for CRASP-1-MBP, (26.9 kDa for CRASP-1 and 42.4 kDa for MBP) in size corresponding to Crasp1 by Western blotting in the appropriate cell lysate or extract. Complement Regulator-Acquiring Surface Protein 1 was tested in SDS-page and western blot.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: CRASP-1

Alternative Name: Crasp1

Background:

Synonyms: control protein, Complement regulator acquiring protein 1, Borrelia burgdorferi CRASP-1

Background: CRASP-1, or Complement Regulator-Acquiring Surface Protein 1, is a multifunctional protein of Lyme disease-causing B. burgdorferi that binds to several human extracellular matrix proteins and plasminogen, including factor H (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism and may allow dissemination of B. burgdorferi in the host. B. burgdorferi spirochetes express up to 5 complement regulator-acquiring surface proteins. Multiple copies of sequences analagous to CRASP-1 genes have been detected in Borrelia plasmids. Borrelia species contain a large number of plasmids, of linear and circular, some of which appear to repeat sequences or contain fragments of other genes. These regions may serve as potentially usable information for the survival of Borrelia in its multiple environments during its life cycle. In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure typical of RNA genes. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

Gene ID: 1194383

NCBI Accession: WP_010890397

UniProt: Q66ZC1