Collagen IV Protein

Catalog No. ABIN5624595

Supplier Product No.: 001-001-106

Native Collagen IV protein expressed in Cow.

Quick Overview for Collagen IV Protein (ABIN5624595)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Protein Type: Native

Origin: Cow

Source: Cow

Application: ELISA, Immunohistochemistry (IHC), SDS-PAGE (SDS), Western Blotting (WB)

Product Details

Supplier Product No.: 001-001-106

Supplier: Rockland

Purpose: Bovine Collagen Type IV

Characteristics: Type IV collagen, collagen 4, bovine collagen

Purification: Bovine Collagen Type IV has been prepared from Bovine Placenta and is chromatographically and immunologically pure.  Bovine Collagen Type IV reacts with anti-Collagen Type IV.  Reaction with Rockland's anti-Collagen I, II, III, V or VI is negligible.

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Bovine Collagen Type IV has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.5 M Acetic Acid

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Bovine Collagen Type IV is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Storing dilutions is not recommended.

Expiry Date: 6 months

Target Details

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV

Background: Background: Bovine Collagen Type IV is one of a family of proteins found particularly in the flesh and connective tissues of mammals (approximately one-third of the body's total protein). Over two dozen types of collagen have been described, though Type IV in particular is involved in forming the foundation of cellular basement membranes, the lens of the eye, and within the capillaries of the glomeruli and nephron in the kidneys.

Gene ID: 407107

UniProt: Q28084