Collagen IV Protein

Catalog No. ABIN5624596

Supplier Product No.: 009-001-106

This Native Collagen IV protein is produced in Human.

Quick Overview for Collagen IV Protein (ABIN5624596)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Protein Type: Native

Origin: Human

Source: Human

Application: ELISA, Immunohistochemistry (IHC), SDS-PAGE (SDS), Western Blotting (WB)

Product Details

Supplier Product No.: 009-001-106

Supplier: Rockland

Purpose: Human Collagen Type IV

Characteristics: Type IV collagen, collagen 4, human collagen, Collagen alpha-4 (IV) chain

Purification: This product has been prepared from human placenta and is chromatographically and immunologically pure.  This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.  This product reacts with anti-Collagen Type IV.  Reaction with Rockland's anti-Collagen I, II, III, V or VI is negligible.

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Collagen type IV has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.5 M Acetic Acid

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: COLLAGEN IV

Background: Background: Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.

UniProt: P02462