Collagen Type V Protein

Catalog No. ABIN5624597

Supplier Product No.: 001-001-107

This Native Collagen Type V protein is expressed in Cow.

Quick Overview for Collagen Type V Protein (ABIN5624597)

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Protein Type: Native

Origin: Cow

Source: Cow

Application: ELISA, Immunohistochemistry (IHC), SDS-PAGE (SDS), Western Blotting (WB)

Product Details

Supplier Product No.: 001-001-107

Supplier: Rockland

Purpose: Bovine Collagen Type V

Characteristics: Extracellular Matrix Protein

Purification: Bovine COLLAGEN Type V has been prepared from Bovine Placenta and is chromatographically and immunologically pure.  This product reacts with anti-Collagen Type V.  Reaction with Rockland's anti-Collagen I, II, III, IV or VI is negligible.

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Bovine COLLAGEN Type V has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.5 M Acetic Acid

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Alternative Name: Collagen Type V

Background: Background: In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6 % of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.