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Flagellin Protein (FliC)

FliC Origin: Borrelia burgdorferi Host: Escherichia coli (E. coli) Recombinant ELISA, WB, SDS, CP
Rockland
Catalog No. ABIN5624606
Supplier Product No.: 000-001-c14
  • Target See all Flagellin (FliC) Proteins
    Flagellin (FliC)
    Protein Type
    Recombinant
    Origin
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Borrelia burgdorferi
    Source
    • 7
    • 5
    • 2
    Escherichia coli (E. coli)
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Control Peptide (CP)
    Supplier Product No.
    000-001-c14
    Supplier
    Rockland
    Purpose
    Flagellin Control Protein
    Purification
    Flagellin is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Flagellin and was estimated to be greater than 90% pure.
    Sterility
    Sterile filtered
    Top Product
    Discover our top product FliC Protein
  • Application Notes

    Application Note: Flagellin is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 76.3 kDa Flagellin-MBP, (33.9 kDa for Flagellin and 42.4 kDa for MBP) in size corresponding to Flagellin by Western blotting in the appropriate cell lysate or extract. Flagellin Protein was tested in SDS-page and western blot.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: Lateral Flow Assay: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
    Expiry Date
    6 months
  • Target
    Flagellin (FliC)
    Alternative Name
    Flagellin (FliC Products)
    Background

    Synonyms: 41 kDa antigen, Borrelia burgdorferi p41, fla, Flagellar filament 41 kDa core protein, Bacterial flagellin, control protein

    Background: Flagellin is a protein found in the hollow cylinder forming the filament in bacterial flagellum. Its structure is helical, which is important for its function. Studies comparing a flagellate Borrelia to flagellated indicate that the flagella have a role in the invasion of human tissue. The N- and C-termini of flagellin form the inner core of the flagellar filament, and the central portion of the protein makes up the outer surface. While the terminus of the protein is quite similar between all bacterial flagellins, the central portion is variable. The flagellin genes are highly conserved among the different Borrelia species. Mammals often have acquired immune responses (T-cell and antibody responses) to flagellated bacterium. Some bacteria are able to switch between multiple flagellin genes in order to evade this response. Borrelia burgdorferi, the spirochete that is associated with Lyme Disease, may use this tactic when challenging mammals with infection. Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids, in low copy number. This suggests that initiation of DNA replication and partitioning are carefully controlled during the cell division cycle. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host, i.e., changes in environment as the spirochete migrates from the tick's midgut to its salivary glands to the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

    Gene ID
    7106737
    NCBI Accession
    WP_002661938
    UniProt
    P11089
    Pathways
    Inflammasome
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