YBX1 Protein

Catalog No. ABIN5624618

Supplier Product No.: 000-001-c16

Product Details

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Protein Type: Recombinant

Origin: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Control Peptide (CP)

Supplier Product No.: 000-001-c16

Supplier: Rockland

Purpose: DbpB Control Protein

Purification: DbpB is a fusion protein synthesized with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified DbpB and was estimated to be greater than 90% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: DbpB protein has been tested by SDS-PAGE and Western blotting and is suitable as a control in other immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 60.3 kDa for DbpB-MBP, (17.9 kDa for DbpB and 42.4 kDa for MBP) in size corresponding to DbpB by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Alternative Name: DbpB (YBX1 Products)

Background:

Synonyms: Decorin-binding protein B, Borrelia burgdorferi DbpB, control protein, DbpB Control Protein

Background: Decorin-binding protein B, or DbpB, binds to decorin, which may mediate the adherence of B. burgdorferi to collagen fibers in skin and other tissues. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix enabling bacteria to colonize in mammalian tissues. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Borrelia burgdorferi can colonize multiple tissues, and is capable of attachment to diverse cell types. The expression of decorin-binding protein (Dbp) A and/or DbpB, two B. burgdorferi surface proteins that bind GAGs, is sufficient to convert a high-passage nonadherent B. burgdorferi strain into one that efficiently binds 293 epithelial cells. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

Gene ID: 1194341

NCBI Accession: WP_010890381

UniProt: P0CL68

Pathways: Regulation of Muscle Cell Differentiation