PARP1 Protein (N-Term)

Catalog No. ABIN5624619

Supplier Product No.: 009-001-gm8

PARP1 protein expressed in Escherichia coli (E. coli).

Quick Overview for PARP1 Protein (N-Term) (ABIN5624619)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Origin: Human

Source: Escherichia coli (E. coli)

Application: Control (Ct)

Product Details

Protein Characteristics: N-Term

Supplier Product No.: 009-001-gm8

Supplier: Rockland

Purification: PARP1 (N-term ZF1) is an N-terminus His-Tag recombinant protein expressed in E.coli that corresponds to a fragment of the human PARP1 zinc finger domain. Analysis by SDS-PAGE and Coomassie staining resulted in ~13 kDa MW band and estimated purity ≥95%.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: PARP1 (N-term ZF1) is suitable as a control for immunological assays that use Anti-PARP1-ZF (RABBIT) Antibody (200-401-GM8). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to PARP-1 by western blotting.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 % (v/v) Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable 2 - 3 weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location