Bovine Coronavirus Spike Protein (AA 326-540, partial) (His tag)

Catalog No. ABIN5710878

Recombinant Bovine Coronavirus Spike protein expressed in Escherichia coli (E. coli).

Quick Overview for Bovine Coronavirus Spike Protein (AA 326-540, partial) (His tag) (ABIN5710878)

Target: Bovine Coronavirus Spike (BCoV S) (Bovine Coronavirus Spike Protein (BCoV S))

Protein Type: Recombinant

Origin: Bovine Coronavirus (BCoV)

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 326-540, partial

Purification tag / Conjugate: This Bovine Coronavirus Spike protein is labelled with His tag.

Sequence: PNLPDCNIEA WLNDKSVPSP LNWERKTFSN CNFNMSSLMS FIQADSFTCN NIEAAKIYGM CFSSITIDKF AIPNGRKVDL QLGNLGYLQS FNYRIDTTAA SCQLYYNLPA ANVSVSRFNP STWNRRFGFT EQSVFKPQPV GVFTHHDVVY AQHCFKAPTN FCPCKLDGSL CVGNGPGIDA GYKNSGIGTC PAGTNYLTCH NAAQCDCLCT PDPIT

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: Bovine Coronavirus Spike (BCoV S) (Bovine Coronavirus Spike Protein (BCoV S))

Alternative Name: Bovine Corona Virus Peplomer Protein

Target Type: Viral Protein

Background: S1 attaches the virion to the cell mbrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell mbrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell mbranes .

Molecular Weight: 27.7 kDa

UniProt: P25194