Gag (AA 216-478) protein (GST tag)

Catalog No. ABIN5710990

This Recombinant protein is produced in Escherichia coli (E. coli).

Quick Overview for Gag (AA 216-478) protein (GST tag) (ABIN5710990)

Target: Gag

Protein Type: Recombinant

Origin: Moloney Murine Leukemia Virus (MoMuLV)

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 216-478

Purification tag / Conjugate: GST tag

Sequence: PLRAGGNGQL QYWPFSSSDL YNWKNNNPSF SEDPGKLTAL IESVLITHQP TWDDCQQLLG TLLTGEEKQR VLLEARKAVR GDDGRPTQLP NEVDAAFPLE RPDWDYTTQA GRNHLVHYRQ LLLAGLQNAG RSPTNLAKVK GITQGPNESP SAFLERLKEA YRRYTPYDPE DPGQETNVSM SFIWQSAPDI GRKLERLEDL KNKTLGDLVR EAEKIFNKRE TPEEREERIR RETEEKEERR RTEDEQKEKE RDRRRHREMS KLL

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: Gag

Target Type: Viral Protein

Background: Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release.

Molecular Weight: 58 kDa

UniProt: P03332