SMARCB1 Protein (AA 1-494, full length) (His-SUMO Tag)

Catalog No. ABIN5711335

This Recombinant SMARCB1 protein is produced in Escherichia coli (E. coli).

Quick Overview for SMARCB1 Protein (AA 1-494, full length) (His-SUMO Tag) (ABIN5711335)

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: full length, AA 1-494

Purification tag / Conjugate: This SMARCB1 protein is labelled with His-SUMO Tag.

Sequence: MATEAQSEGE VPARESGRSD AICSFVICND SSLRGQPIIF NPDFFVEKLR HEKPEIFTEL VVSNITRLID LPGTELAQLM GEVDLKLPGG AGPASGFFRS LMSLKRKEKG VIFGSPLTEE GIAQIYQLIE YLHKNLRVEG LFRVPGNSVR QQILRDALNN GTDIDLESGE FHSNDVATLL KMFLGELPEP LLTHKHFNAH LKIADLMQFD DKGNKTNIPD KDRQIEALQL LFLILPPPNR NLLKLLLDLL YQTAKKQDKN KMSAYNLALM FAPHVLWPKN VTANDLQENI TKLNSGMAFM IKHSQKLFKA PAYIRECARL HYLGSRTQAS KDDLDLIASC HTKSFQLAKS QKRNRVDSCP HQEETQHHTE EALRELFQHV HDMPESAKKK QLIRQFNKQS LTQTPGREPS TSQVQKRARS RSFSGLIKRK VLGNQMMSEK KKKNPTPESV AIGELKGTSK ENRNLLFSGS PAVTMTPTRL KWSEGKKEGK KGFL

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Alternative Name: SNF5

Background: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-rodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the rodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin rodeling complex (npBAF complex) and the neuron-specific chromatin rodeling complex (nBAF complex). During neural development a switch from a st/progenitor to a post-mitotic chromatin rodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural st/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural st cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth . Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.

Molecular Weight: 71.7 kDa

UniProt: Q12824