SART3 Protein (AA 600-900, partial) (His-SUMO Tag)

Catalog No. ABIN5711337

This Recombinant SART3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SART3 Protein (AA 600-900, partial) (His-SUMO Tag) (ABIN5711337)

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 600-900, partial

Purification tag / Conjugate: This SART3 protein is labelled with His-SUMO Tag.

Sequence: QRKRARAEKK ALKKKKKIRG PEKRGADEDD EKEWGDDEEE QPSKRRRVEN SIPAAGETQN VEVAAGPAGK CAAVDVEPPS KQKEKAASLK RDMPKVLHDS SKDSITVFVS NLPYSMQEPD TKLRPLFEAC GEVVQIRPIF SNRGDFRGYC YVEFKEEKSA LQALEMDRKS VEGRPMFVSP CVDKSKNPDF KVFRYSTSLE KHKLFISGLP FSCTKEELEE ICKAHGTVKD LRLVTNRAGK PKGLAYVEYE NESQASQAVM KMDGMTIKEN IIKVAISNPP QRKVPEKPET RKAPGGPMLL P

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Alternative Name: SART3

Background: U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassbly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation . Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assbly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites . May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassbly . May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair . May play a role in hatopoiesis probably through transcription regulation of specific genes including MYC .

Molecular Weight: 49.82 kDa

UniProt: Q15020

Pathways: Ribonucleoprotein Complex Subunit Organization