VP4 (AA 247-479) protein (His tag)

Catalog No. ABIN5714038

This Recombinant protein is produced in Yeast.

Quick Overview for VP4 (AA 247-479) protein (His tag) (ABIN5714038)

Target: VP4

Protein Type: Recombinant

Origin: Rotavirus A

Source: Yeast

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 247-479

Purification tag / Conjugate: His tag

Sequence: AQVSEDIIIS KTSLWKEMQY NRDIIIRFKF NNSIIKLGGL GYKWSEISFK AANYQYNYLR DGEQVTAHTT CSVNGVNNFS YNGGLLPTHF SISRYEVIKE NSYVYVDYWD DSQAFRNMVY VRSLAANLNS VKCSGGNYNF QMPVGAWPVM SGGAVSLHFA GVTLSTQFTD FVSLNSLRFR FSLTVEEPPF SILRTRVSGL YGLPASNPNS GHEYYEIAGR FSLISLVPSN DDY

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: VP4

Target Type: Viral Protein

Background: Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. According to the considered strain, VP4 ses to essentially target sialic acid and/or the integrin heterodimer ITGA2/ITGB1 .Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a mbrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment .VP8* forms the head of the spikes. It is the viral hagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact .

Molecular Weight: 28.3 kDa

UniProt: P11200