Mago Nashi Homolog 2 Protein (AA 1-148) (His tag)

Catalog No. ABIN5853006

Recombinant Mago Nashi Homolog 2 protein expressed in Escherichia coli (E. coli).

Quick Overview for Mago Nashi Homolog 2 Protein (AA 1-148) (His tag) (ABIN5853006)

Target: Mago Nashi Homolog 2 (MAGOHB) (Mago-Nashi Homolog B (MAGOHB))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-148

Purification tag / Conjugate: This Mago Nashi Homolog 2 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAVASDF YLRYYVGHKG KFGHEFLEFE FRPDGKLRYA NNSNYKNDVM IRKEAYVHKS VMEELKRIID DSEITKEDDA LWPPPDRVGR QELEIVIGDE HISFTTSKIG SLIDVNQSKD PEGLRVFYYL VQDLKCLVFS LIGLHFKIKP I

Purification: > 90% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT 30 % glycerol, 0.15M NaCl

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Mago Nashi Homolog 2 (MAGOHB) (Mago-Nashi Homolog B (MAGOHB))

Alternative Name: MAGOHB

Background: MAGOHB is involved in mRNA splicing and in the nonsense-mediated decay (NMD) pathway. Also, it is interacts with RBM8A and is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, uPF3B, uPF2 and ALYREF/THOC4. Recombinant human MAGOHB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 19.7 kDa (171aa) confirmed by MALDI-TOF

NCBI Accession: NP_060518