TCEA2 Protein (AA 1-299) (His tag)
Quick Overview for TCEA2 Protein (AA 1-299) (His tag) (ABIN5853007)
Target
See all TCEA2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-299
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Purification tag / Conjugate
- This TCEA2 protein is labelled with His tag.
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Sequence
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MMGKEEE IARIARRLDK MVTKKSAEGA MDLLRELKAM PITLHLLQST RVGMSVNALR KQSSDEEVIA LAKSLIKSWK KLLDASDAKA RERGRGMPLP TSSRDASEAP DPSRKRPELP RAPSTPRITT FPPVPVTCDA VRNKCREMLT AALQTDHDHV AIGADCERLS AQIEECIFRD VGNTDMKYKN RVRSRISNLK DAKNPDLRRN VLCGAITPQQ IAVMTSEEMA SDELKEIRKA MTKEAIREHQ MARTGGTQTD LFTCGKCRKK NCTYTQVQTR SSDEPMTTFV VCNECGNRWK FC -
Purification
- > 85% by SDS-PAGE
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Transcription Elongation Factor A2 (TCEA2) (AA 1-299) protein (His tag)
custom-made TCEA2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Transcription Elongation Factor A2 (TCEA2) (AA 1-299) protein (His tag)custom-made TCEA2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Transcription Elongation Factor A2 (TCEA2) (AA 1-299) protein (His-IF2DI Tag)TCEA2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Transcription Elongation Factor A2 (TCEA2) (AA 1-299) protein (GST tag)TCEA2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Transcription Elongation Factor A2 (TCEA2) (AA 1-299), (full length) protein (His-SUMO Tag)TCEA2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 30 % glycerol, 0.25M NaCl
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- TCEA2 (Transcription Elongation Factor A2 (TCEA2))
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Alternative Name
- TCEA2
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Background
- TCEA2 is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human TCEA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 36 kDa (322aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_003186
Target
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