HNRNPK Protein (AA 1-276) (His tag)
Quick Overview for HNRNPK Protein (AA 1-276) (His tag) (ABIN5853065)
Target
See all HNRNPK ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-276
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Purification tag / Conjugate
- This HNRNPK protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMETEQPE ETFPNTETNG EFGKRPAEDM EEEQAFKRSR NTDEMVELRI LLQSKNAGAV IGKGGKNIKA LRTDYNASVS VPDSSGPERI LSISADIETI GEILKKIIPT LEEGLQLPSP TATSQLPLES DAVECLNYQH YKGSDFDCEL RLLIHQSLAG GIIGVKGAKI KELRENTQTT IKLFQECCPH STDRVVLIGG KPDRVVECIK IILDLISESP IKGRAQPYDP NFYDETYDYG GFTMMFDDRR GRPVGFPMRG RGGFDRMPPG RGGRPMPPS
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Purification
- > 95% by SDS-PAGE
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Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK) (AA 1-463) protein (His tag)
custom-made HNRNPK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK) (AA 1-463) protein (His tag)custom-made HNRNPK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK) (AA 1-463) protein (GST tag)HNRNPK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK) (AA 3-459), (partial) protein (GST tag)HNRNPK Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)HNRNPK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))
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Alternative Name
- HNRNPK
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Background
- HNRNPK belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. HNRNPK is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. Recombinant human HNRNPK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 33 kDa (299aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_002131
Target
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