UFD1L Protein (AA 1-307) (His tag)

Catalog No. ABIN5853130

Product Details

Target: UFD1L (Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L))

Protein Type: Recombinant

Protein Characteristics: AA 1-307

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UFD1L protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMFSFNMF DHPIPRVFQN RFSTQYRCFS VSMLAGPNDR SDVEKGGKII MPPSALDQLS RLNITYPMLF KLTNKNSDRM THCGVLEFVA DEGICYLPHW MMQNLLLEEG GLVQVESVNL QVATYSKFQP QSPDFLDITN PKAVLENALR NFACLTTGDV IAINYNEKIY ELRVMETKPD KAVSIIECDM NVDFDAPLGY KEPERQVQHE ESTEGEADHS GYAGELGFRA FSGSGNRLDG KKKGVEPSPS PIKPGDIKRG IPNYEFKLGK ITFIRNSRPL VKKVEEDEAG GRFVAFSGEG QSLRKKGRKP

Purity: > 85 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.1M NaCl, 30 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: UFD1L (Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L))

Alternative Name: uFD1L (UFD1L Products)

Synonyms: UFD1 Protein, Ufd1 Protein, ufd1 Protein, wu:fc55f04 Protein, zgc:92341 Protein, Ufd1l Protein, ubiquitin recognition factor in ER associated degradation 1 Protein, ubiquitin recognition factor in ER-associated degradation 1 Protein, ubiquitin fusion degradation 1 like (yeast) Protein, ubiquitin recognition factor in ER associated degradation 1 S homeolog Protein, ubiquitin fusion degradation protein 1 homolog Protein, Ubiquitin Fusion Degradation (yeast UFD homolog) Protein, UFD1 Protein, Ufd1 Protein, UFD1L Protein, ufd1.S Protein, ufd1l Protein, ufd1 Protein, EHI_125920 Protein, LOC100166745 Protein, LOC100635687 Protein, LOC100648660 Protein, ufd-1 Protein

Background: UFD1L forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. Recombinant human uFD1L protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 36.9 kDa (330aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

NCBI Accession: NP_005650

UniProt: Q92890