UFD1L Protein (AA 1-307) (His tag)
Quick Overview for UFD1L Protein (AA 1-307) (His tag) (ABIN5853130)
Target
See all UFD1L ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-307
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Purification tag / Conjugate
- This UFD1L protein is labelled with His tag.
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Sequence
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MFSFNMF DHPIPRVFQN RFSTQYRCFS VSMLAGPNDR SDVEKGGKII MPPSALDQLS RLNITYPMLF KLTNKNSDRM THCGVLEFVA DEGICYLPHW MMQNLLLEEG GLVQVESVNL QVATYSKFQP QSPDFLDITN PKAVLENALR NFACLTTGDV IAINYNEKIY ELRVMETKPD KAVSIIECDM NVDFDAPLGY KEPERQVQHE ESTEGEADHS GYAGELGFRA FSGSGNRLDG KKKGVEPSPS PIKPGDIKRG IPNYEFKLGK ITFIRNSRPL VKKVEEDEAG GRFVAFSGEG QSLRKKGRKP -
Purification
- > 85% by SDS-PAGE
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Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L) (AA 1-307) protein (His tag)
custom-made UFD1L Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L) (AA 1-307) protein (GST tag)UFD1L Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L) (AA 1-307) protein (His-IF2DI Tag)UFD1L Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UFD1L Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UFD1L Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 30 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- UFD1L (Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L))
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Alternative Name
- UFD1L
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Background
- UFD1L forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. Recombinant human UFD1L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 36.9 kDa (330aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
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NCBI Accession
- NP_005650
Target
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