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NOB1 Protein (AA 1-412) (His tag)

NOB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % by SDS - PAGE SDS
Catalog No. ABIN5853244
  • Target See all NOB1 Proteins
    NOB1 (RNA-Binding Protein NOB1 (NOB1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-412
    Origin
    • 3
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 3
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This NOB1 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS)
    Sequence
    MGSSHHHHHH SSGLVPRGSH MGSMAPVEHV VADAGAFLRH AALQDIGKNI YTIREVVTEI RDKATRRRLA VLPYELRFKE PLPEYVRLVT EFSKKTGDYP SLSATDIQVL ALTYQLEAEF VGVSHLKQEP QKVKVSSSIQ HPETPLHISG FHLPYKPKPP QETEKGHSAC EPENLEFSSF MFWRNPLPNI DHELQELLID RGEDVPSEEE EEEENGFEDR KDDSDDDGGG WITPSNIKQI QQELEQCDVP EDVRVGCLTT DFAMQNVLLQ MGLHVLAVNG MLIREARSYI LRCHGCFKTT SDMSRVFCSH CGNKTLKKVS VTVSDDGTLH MHFSRNPKVL NPRGLRYSLP TPKGGKYAIN PHLTEDQRFP QLRLSQKARQ KTNVFAPDYI AGVSPFVEND ISSRSATLQV RDSTLGAGRR RLNPNASRKK FVKKR
    Purity
    > 80 % by SDS - PAGE
    Top Product
    Discover our top product NOB1 Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Denatured

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M urea, 10 % glycerol
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    NOB1 (RNA-Binding Protein NOB1 (NOB1))
    Alternative Name
    NOB1 (NOB1 Products)
    Synonyms
    MEE6.26 Protein, MEE6_26 Protein, Nob1p Protein, fc27e05 Protein, si:ch73-167i17.3 Protein, wu:fc27e05 Protein, ART-4 Protein, MST158 Protein, NOB1P Protein, PSMD8BP1 Protein, 1700021I09Rik Protein, Psmd8bp1 Protein, NIN1/PSMD8 binding protein 1 homolog Protein, RNA-binding NOB1-like protein Protein, RNA-binding protein nob1 Protein, RNA-binding protein NOB1 Protein, NIN1/RPN12 binding protein 1 homolog S homeolog Protein, NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) Protein, NIN1/RPN12 binding protein 1 homolog Protein, NOB1 Protein, AT5G41190 Protein, EDI_130960 Protein, CpipJ_CPIJ012739 Protein, BDBG_01281 Protein, PAAG_04204 Protein, PITG_13300 Protein, nob1.S Protein, nob1 Protein, Nob1 Protein
    Background
    In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. Recombinant human NOB1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
    Molecular Weight
    49.1 kDa (435aa)
    NCBI Accession
    NP_054781
    UniProt
    Q9ULX3
    Pathways
    Protein targeting to Nucleus
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