EIF3F Protein (AA 1-357) (His tag)
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- Target See all EIF3F Proteins
- EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-357
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EIF3F protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product EIF3F Protein
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Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 1-357) protein (GST tag)
EIF3F Origin: Human Host: Wheat germ Recombinant ELISA, AA, AP, WB
Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 2-250), (partial) protein (GST tag)EIF3F Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 1-323), (partial) protein (GST tag)EIF3F Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) protein (Myc-DYKDDDDK Tag)EIF3F Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.4M urea
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))
- Alternative Name
- EIF3F (EIF3F Products)
- Background
- EIF3F is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AuG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3F protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 40.0kDa (380aa)
- NCBI Accession
- NP_003745
- UniProt
- O00303
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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