ARPP19 Protein (AA 1-112) (His tag)

Catalog No. ABIN5853398

This Recombinant ARPP19 protein is produced in Escherichia coli (E. coli).

Quick Overview for ARPP19 Protein (AA 1-112) (His tag) (ABIN5853398)

Target: ARPP19 (cAMP-Regulated Phosphoprotein, 19kDa (ARPP19))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-112

Purification tag / Conjugate: This ARPP19 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSAEVPE AASAEEQKEM EDKVTSPEKA EEAKLKARYP HLGQKPGGSD FLRKRLQKGQ KYFDSGDYNM AKAKMKNKQL PTAAPDKTEV TGDHIPTPQD LPQRKPSLVA SKLAG

Purification: > 90% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ARPP19 (cAMP-Regulated Phosphoprotein, 19kDa (ARPP19))

Alternative Name: ARPP19

Background: CAMP-regulated phosphoprotein 19, also known as ARPP19, is protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-62 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. This protein may indirectly enhance GAP-43 expression. Recombinant human ARPP19 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 14.7 kDa (135aa) confirmed by MALDI-TOF

NCBI Accession: NP_006619

Pathways: Regulation of Carbohydrate Metabolic Process