CA14 Protein (AA 16-290) (His tag)
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- Target See all CA14 Proteins
- CA14 (Carbonic Anhydrase XIV (CA14))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 16-290
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CA14 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSADGGQHW TYEGPHGQDH WPASYPECGN NAQSPIDIQT DSVTFDPDLP ALQPHGYDQP GTEPLDLHNN GHTVQLSLPS TLYLGGLPRK YVAAQLHLHW GQKGSPGGSE HQINSEATFA ELHIVHYDSD SYDSLSEAAE RPQGLAVLGI LIEVGETKNI AYEHILSHLH EVRHKDQKTS VPPFNLRELL PKQLGQYFRY NGSLTTPPCY QSVLWTVFYR RSQISMEQLE KLQGTLFSTE EEPSKLLVQN YRALQPLNQR MVFASFIQAG SSYTTGEM
- Purity
- > 85 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product CA14 Protein
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Carbonic Anhydrase XIV (CA14) (AA 16-290) protein (His tag)
CA14 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Carbonic Anhydrase XIV (CA14) (AA 1-337) protein (GST tag)CA14 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Carbonic Anhydrase XIV (CA14) (AA 16-337) protein (rho-1D4 tag)Crystallography grade CA14 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Carbonic Anhydrase XIV (CA14) (AA 16-337) protein (rho-1D4 tag)Crystallography grade CA14 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CA14 (Carbonic Anhydrase XIV (CA14))
- Alternative Name
- CA14 (CA14 Products)
- Synonyms
- zgc:123188 Protein, AW536446 Protein, Ca14 Protein, CAXiV Protein, carbonic anhydrase XIV Protein, carbonic anhydrase 14 Protein, CA14 Protein, ca14 Protein, Car14 Protein
- Background
- Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA14 is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII, however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. Recombinant human CA14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 33.2kDa (298aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_036245
- UniProt
- Q9ULX7
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