ATP5F1 Protein (AA 83-256) (His tag)

Catalog No. ABIN5853489

Product Details

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Protein Type: Recombinant

Protein Characteristics: AA 83-256

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ATP5F1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSLILYALS KEIYVISAET FTALSVLGVM VYGIKKYGPF VADFADKLNE QKLAQLEEAK QASIQHIQNA IDTEKSQQAL VQKRHYLFDV QRNNIAMALE VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST QQEKETIAKC IADLKLLAKK AQAQPVM

Purity: > 80 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M urea, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Alternative Name: ATP5F1 (ATP5F1 Products)

Synonyms: PIG47 Protein, C76477 Protein, wu:fb59g11 Protein, wu:fj08b08 Protein, zgc:101887 Protein, ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 Protein, ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 S homeolog Protein, ATP synthase F(0) complex subunit B1, mitochondrial Protein, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 Protein, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1 Protein, ATP5F1 Protein, atp5f1.S Protein, atp5f1 Protein, LOC479901 Protein, Atp5f1 Protein

Background: ATP5F1 is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). Recombinant human ATP5F1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 22.6 kDa (197aa)

NCBI Accession: NP_001679

UniProt: P24539

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process