LECT1 Protein (AA 214-334) (His tag)

Catalog No. ABIN5853498

This Recombinant LECT1 protein is produced in Escherichia coli (E. coli).

Quick Overview for LECT1 Protein (AA 214-334) (His tag) (ABIN5853498)

Target: LECT1 (Leukocyte Cell Derived Chemotaxin 1 (LECT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 % by SDS - PAGE

Product Details

Protein Characteristics: AA 214-334

Purification tag / Conjugate: This LECT1 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSREVVRKI VPTTTKRPHS GPRSNPGAGR LNNETRPSVQ EDSQAFNPDN PYHQQEGESM TFDPRLDHEG ICCIECRRSY THCQKICEPL GGYYPWPYNY QGCRSACRVI MPCSWWVARI LGMV

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M uREA, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: LECT1 (Leukocyte Cell Derived Chemotaxin 1 (LECT1))

Alternative Name: LECT1

Background: LECT1 is a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Recombinant human LECT1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 16.3kDa (144aa)

NCBI Accession: NP_008946

UniProt: O75829