Cathepsin E Protein (CTSE) (AA 57-363) (His tag)

Catalog No. ABIN5853580

This Recombinant Cathepsin E protein is expressed in Escherichia coli (E. coli).

Quick Overview for Cathepsin E Protein (CTSE) (AA 57-363) (His tag) (ABIN5853580)

Target: Cathepsin E (CTSE)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 85 % by SDS - PAGE

Product Details

Protein Characteristics: AA 57-363

Purification tag / Conjugate: This Cathepsin E protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSTESCSMD QSAKEPLINY LDMEYFGTIS IGSPPQNFTV IFDTGSSNLW VPSVYCTSPA CKTHSRFQPS QSSTYSQPGQ SFSIQYGTGS LSGIIGADQV SVEGLTVVGQ QFGESVTEPG QTFVDAEFDG ILGLGYPSLA VGGVTPVFDN MMAQNLVDLP MFSVYMSSNP EGGAGSELIF GGYDHSHFSG SLNWVPVTKQ AYWQIALDNM LWSVPTLTSC RMSPSPLTES PIPSAQLPTP YWTSWMECSS AAVAFKDLTS TLQLGPSGSW GMSSFDSFTQ SLTVGITVWD WPQQSPKEGP CVCACLSDRP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M urea, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Cathepsin E (CTSE)

Alternative Name: CTSE

Background: CTSE is a gastric aspartyl protease that functions as a disulfide-linked homodimer. CTSE, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. Recombinant human CTSE protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 35.4 kDa (330aa)

NCBI Accession: NP_683865

UniProt: P14091