Calpain S1 Protein (AA 84-268) (His tag)
Quick Overview for Calpain S1 Protein (AA 84-268) (His tag) (ABIN5853704)
Target
See all Calpain S1 (CAPNS1) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 84-268
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Purification tag / Conjugate
- This Calpain S1 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG TGQIQVNIQE WLQLTMYS
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Purification
- > 95% by SDS-PAGE
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Calpain, Small Subunit 1 (CAPNS1) (AA 1-268) protein (His tag)
custom-made CAPNS1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Calpain, Small Subunit 1 (CAPNS1) (AA 1-268) protein (GST tag)CAPNS1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Calpain, Small Subunit 1 (CAPNS1) (AA 1-268), (full length) protein (His-SUMO Tag)CAPNS1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Calpain, Small Subunit 1 (CAPNS1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CAPNS1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calpain, Small Subunit 1 (CAPNS1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CAPNS1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Calpain S1 (CAPNS1) (Calpain, Small Subunit 1 (CAPNS1))
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Alternative Name
- CAPNS1
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Background
- Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Recombinant human CAPNS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 23.8 kDa (208aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001740
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Pathways
- Apoptosis
Target
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