PELI2 Protein (AA 1-420) (His tag)
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- Target See all PELI2 Proteins
- PELI2 (Pellino Homolog 2 (Drosophila) (PELI2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-420
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PELI2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMFSPGQE EHCAPNKEPV KYGELVVLGY NGALPNGDRG RRKSRFALYK RPKANGVKPS TVHVISTPQA SKAISCKGQH SISYTLSRNQ TVVVEYTHDK DTDMFQVGRS TESPIDFVVT DTISGSQNTD EAQITQSTIS RFACRIVCDR NEPYTARIFA AGFDSSKNIF LGEKAAKWKN PDGHMDGLTT NGVLVMHPRG GFTEESQPGV WREISVCGDV YTLRETRSAQ QRGKLVESET NVLQDGSLID LCGATLLWRT ADGLFHTPTQ KHIEALRQEI NAARPQCPVG LNTLAFPSIN RKEVVEEKQP WAYLSCGHVH GYHNWGHRSD TEANERECPM CRTVGPYVPL WLGCEAGFYV DAGPPTHAFT PCGHVCSEKS AKYWSQIPLP HGTHAFHAAC PFCATQLVGE QNCIKLIFQG PID
- Purity
- > 80 % by SDS - PAGE
- Top Product
- Discover our top product PELI2 Protein
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Pellino Homolog 2 (Drosophila) (PELI2) (AA 1-420) protein (GST tag)
PELI2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Pellino Homolog 2 (Drosophila) (PELI2) (AA 1-420) protein (Strep Tag)Crystallography grade PELI2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Pellino Homolog 2 (Drosophila) (PELI2) (AA 1-419) protein (Strep Tag)Crystallography grade PELI2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PELI2 (Pellino Homolog 2 (Drosophila) (PELI2))
- Alternative Name
- PELI2 (PELI2 Products)
- Synonyms
- AW047589 Protein, BB129927 Protein, pellino 2 Protein, pellino E3 ubiquitin protein ligase family member 2 Protein, Peli2 Protein, PELI2 Protein
- Background
- PELI2 is an E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. This protein is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. It mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. PELI2 may be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. It can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1. Recombinant human PELI2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 48.8 kDa (443aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_067078
- UniProt
- Q9HAT8
- Pathways
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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