S100A8 Protein (AA 1-93) (His-Myc tag)
Quick Overview for S100A8 Protein (AA 1-93) (His-Myc tag) (ABIN5854609)
Target
See all S100A8 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-93
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Purification tag / Conjugate
- This S100A8 protein is labelled with His-Myc tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHMLTELE KALNSIIDVY HKYSLIKGNF HAVYRDDLKK LLETECPQYI RKKGADVWFK ELDINTDGAV NFQEFLILVI KMGVAAHKKS HEESHKEEQK LISEEDL
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) protein (His tag)
custom-made S100A8 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) protein (GST tag)S100A8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) proteinS100A8 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.1M NaCl
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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Alternative Name
- S100A8
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Background
- S100A8, also known as S100A8 isoform d. S100A8 is a member of the S100 family, EF-hand superfamily of Calcium binding proteins. S100A8 protein is localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Altered expression of this protein is associated with the disease cystic fibrosis. Recombinant human S100A8, fused to His-tag at N-terminus, fused to Myc-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 14.5 kDa (127aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_002955
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Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
Target
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