CD58 Protein (CD58) (AA 29-215) (His tag)
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- Target See all CD58 Proteins
- CD58
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-215
- Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This CD58 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPFSQQIYG VVYGNVTFHV PSNVPLKEVL WKKQKDKVAE LENSEFRAFS SFKNRVYLDT VSGSLTIYNL TSSDEDEYEM ESPNITDTMK FFLYVLESLP SPTLTCALTN GSIEVQCMIP EHYNSHRGLI MYSWDCPMEQ CKRNSTSIYF KMENDLPQKI QCTLSNPLFN TTSSIILTTC IPSSGHSRHR HHHHHH
- Purity
- > 95% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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CD58 (CD58) (AA 29-215) protein (Fc Tag)
CD58 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD58 (CD58) (AA 1-240) protein (GST tag)CD58 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD58 (CD58) (AA 29-215) protein (His tag)CD58 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
CD58 (CD58) (AA 29-215) (Active) protein (Fc Tag)CD58 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- CD58
- Alternative Name
- CD58/LFA-3 (CD58 Products)
- Background
- CD58, also known as lymphocyte function-associated antigen 3 isoform 1, is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. It is an important regulator of immune cell function which occurs as both surface-associated and soluble forms. It is associated with increased risk for multiple sclerosis. Recombinant human CD58, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 22.5 kDa (196aa)
- NCBI Accession
- NP_001770
- UniProt
- P19256
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