Glypican 3 Protein (GPC3) (AA 25-559) (His tag,AVI tag,Biotin)

Catalog No. ABIN5954920

This Recombinant Glypican 3 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for Glypican 3 Protein (GPC3) (AA 25-559) (His tag,AVI tag,Biotin) (ABIN5954920)

Target: Glypican 3 (GPC3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >85 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 25-559

Purification tag / Conjugate: This Glypican 3 protein is labelled with His tag,AVI tag,Biotin.

Sequence: AA 25-559

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

References

Berman, Kelada, Gutsche, Natarajan, Swenson, Fu, Hong, Ho, Choyke, Escorcia: "In Vitro Performance of Published Glypican 3-Targeting Peptides TJ12P1 and L5 Indicates Lack of Specificity and Potency." in: Cancer biotherapy & radiopharmaceuticals, (2019) (PubMed).

Target Details

Target: Glypican 3 (GPC3)

Alternative Name: Glypican 3

Background: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Molecular Weight: 38.1 kDa and 26.4 kDa

NCBI Accession: NP_004475

Pathways: Glycosaminoglycan Metabolic Process