Glypican 3 Protein (GPC3) (AA 25-559) (His tag)

Catalog No. ABIN7274772

Product Details

Target: Glypican 3 (GPC3)

Protein Type: Recombinant

Protein Characteristics: AA 25-559

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This Glypican 3 protein is labelled with His tag.

Purpose: Cynomolgus GPC3/Glypican 3 Protein

Sequence: Gln25-His559

Characteristics: Recombinant Cynomolgus GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus GPC3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Glypican 3 (GPC3)

Alternative Name: GPC3 (GPC3 Products)

Synonyms: GPC3 Protein, sgb Protein, dgsx Protein, sdys Protein, sgbs Protein, oci-5 Protein, sgbs1 Protein, DGSX Protein, GTR2-2 Protein, MXR7 Protein, OCI-5 Protein, SDYS Protein, SGB Protein, SGBS Protein, SGBS1 Protein, Glypican-3 Protein, glypican 3 Protein, gpc3 Protein, GPC3 Protein, Gpc3 Protein

Background: Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Molecular Weight: 61.8 kDa. Due to glycosylation, the protein migrates to 42 kDa, 70-135 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005594665

Pathways: Glycosaminoglycan Metabolic Process