MUC16 Protein (AA 12660-12923) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN5954926

This Recombinant MUC16 protein is expressed in HEK-293 Cells.

Quick Overview for MUC16 Protein (AA 12660-12923) (Fc Tag,AVI tag,Biotin) (ABIN5954926)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 12660-12923

Purification tag / Conjugate: This MUC16 protein is labelled with Fc Tag,AVI tag,Biotin.

Sequence: AA 12660-12923

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: CA125

Background: The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 Molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

Molecular Weight: 56.9 kDa

NCBI Accession: NP_078966