IL1A Protein (AA 115-270)

Catalog No. ABIN7315074

This Recombinant IL1A protein is produced in Escherichia coli (E. coli).

Quick Overview for IL1A Protein (AA 115-270) (ABIN7315074)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 115-270

Purpose: Recombinant Rat IL-1 alpha Protein is produced by E.coli expression system and the target gene encoding Ser115-Ser270 is expressed.

Sequence: MSAPHSFQNN LRYKLIRIVK QEFIMNDSLN QNIYVDMDRI HLKAASLNDL QLEVKFDMYA YSSGGDDSKY PVTLKVSNTQ LFVSAQGEDK PVLLKEIPET PKLITGSETD LIFFWEKINS KNYFTSAAFP ELLIATKEQS QVHLARGLPS MIDFQIS

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS,  pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL1A

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints