IL-20 Protein (AA 25-176) (His tag)

Catalog No. ABIN6239769

This IL-20 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-20 Protein (AA 25-176) (His tag) (ABIN6239769)

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 90 %

Product Details

Protein Characteristics: AA 25-176

Purification tag / Conjugate: This IL-20 protein is labelled with His tag.

Characteristics: Tag location: N-terminal His Tag

Biological Activity Comment: IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which is produced by activated keratinocytes and monocytes. It is accepted that IL20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL20. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The result was shown in Figure 2. It was obvious that human IL20 significantly decreased cell viability of ECV-304 cells.

Application Details

Application Notes: Isoelectric Point: 8.9

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Background: Alternative Names: IL10D, IL10-D, ZCYTO10, Cytokine Zcyto10

Molecular Weight: 21kDa

UniProt: Q9NYY1

Pathways: Protein targeting to Nucleus