IL-6 Protein (AA 30-212) (His tag)
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- Target See all IL-6 (IL6) Proteins
- IL-6 (IL6) (Interleukin 6 (IL6))
- Biological Activity
- Active
- Protein Characteristics
- AA 30-212
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL-6 protein is labelled with His tag.
- Application
- Cell Culture (CC), Activity Assay (AcA)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Current data suggest that direct application of IL-6 on breast cancer cells inhibits proliferation in ER-positive (estrogen- receptor- positive) cells through the Jak/Stat3 pathway. To test the inhibitory effect of IL-6 on proliferation of ER-positive MCF-7 cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of IL-6. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. The inhibitory effect of IL-6 on cell proliferation of MCF-7 cells observed by inverted microscope and detected by CCK-8 was shown in Figure 1 and Figure 2 respectively (Dose-dependent effect was not detected in this case).
- Top Product
- Discover our top product IL6 Protein
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Interleukin 6 (IL6) (AA 30-212) protein (mFc-His Tag)
IL6 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, FACS
Interleukin 6 (IL6) (AA 30-212) proteinIL6 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 6 (IL6) (AA 28-212) protein (His tag)IL6 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 6 (IL6) (AA 29-212) (Active) proteinIL6 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 6 (IL6) (AA 25-211) (Active) protein (His tag)IL6 Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 6 (IL6) (AA 30-212) (Active) proteinIL6 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 6 (IL6) (AA 25-211) protein (Fc Tag)IL6 Origin: Mouse Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 6 (IL6) (AA 30-212) (Active) proteinIL6 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS Active
Interleukin 6 (IL6) (AA 25-211) protein (His tag)IL6 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- Application Notes
- Isoelectric Point: 6.2
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- IL-6 (IL6) (Interleukin 6 (IL6))
- Abstract
- IL6 Products
- Background
- Alternative Names: MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor
- Molecular Weight
- 22/24kDa
- UniProt
- Q90YI0
- Pathways
- TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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