MASP2 Protein (AA 445-683) (His tag)

Catalog No. ABIN6239794

Product Details

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Biological Activity: Active

Protein Characteristics: AA 445-683

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MASP2 protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 97 %

Biological Activity Comment: MASP2 (Mannan-binding lectin serine protease 2) is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. The preproprotein of MASP2 is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease, which is able to associate with MBL2. Thus, a functional binding ELISA assay was constructed to detect the association of rhMASP2 with MBL2. Briefly, rhMASP-2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05%Triton X-100 (pH 7.4). Duplicate samples of 100uL were then transferred to MBL2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MASP-2 pAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MASP2 with MBL2 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of MASP2 with MBL2.

Application Details

Application Notes: Isoelectric Point: 5.7

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Alternative Name: Mannose Associated Serine Protease 2 (MASP2) (MASP2 Products)

Synonyms: MAP19 Protein, MASP-2 Protein, MASP1P1 Protein, sMAP Protein, MAp19 Protein, mannan binding lectin serine peptidase 2 Protein, mannan-binding lectin serine peptidase 2 Protein, MASP2 Protein, Masp2 Protein

Background: Alternative Names: Mannose-binding protein-associated serine protease 2, mannan-Binding Lectin Serine Peptidase 2, MBL-associated serine protease 2

Molecular Weight: 27kDa

UniProt: O00187

Pathways: Complement System