MASP2 Protein (AA 287-686) (His tag)

Catalog No. ABIN7275291

This Recombinant MASP2 protein is produced in Escherichia coli (E. coli).

Quick Overview for MASP2 Protein (AA 287-686) (His tag) (ABIN7275291)

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 287-686

Purification tag / Conjugate: This MASP2 protein is labelled with His tag.

Purpose: Cynomolgus MASP2 Protein

Sequence: Thr287-Phe686

Characteristics: Recombinant Cynomolgus MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe686.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Narsoplimab, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.,Cynomolgus MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Cynomolgus MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris, 200 mM NaCl ( pH 9.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Alternative Name: MASP2

Background: Te MASP2 gene is located on the chromosome 1p36.23-31 and encodes two proteins, MASP-2 and Map198. Both the levels and function of MASP-2 are modulated by the single-nucleotide polymorphisms (SNPs) of the MASP2 gene. Te level of MASP-2 in plasma plays a dual role in the infection and progression of infectious diseases. Te defciency of MASP-2 in plasma has been associated with the susceptibility of HIV. By contrast, the overproduction of MASP-2 may increase the infammatory efect.

Molecular Weight: 46.8 kDa. Due to autocatalytic cleavage, the protein migrates to 21-24 kDa&28-30 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5UJY0

Pathways: Complement System