MASP2 Protein (AA 288-686) (His tag)
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- Target See all MASP2 Proteins
- MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 288-686
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MASP2 protein is labelled with His tag.
- Purpose
- Human MASP2 Protein
- Sequence
- Gly288-Phe686
- Characteristics
- Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-686) protein (His tag)
MASP2 Origin: Cynomolgus Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)MASP2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag,Biotin)MASP2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)MASP2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) protein (Fc Tag)MASP2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 1-686) protein (GST tag)MASP2 Origin: Human Host: Wheat germ Recombinant ELISA, AP, AA, WB
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 445-683) (Active) protein (His tag)MASP2 Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 200 mM NaCl ( pH 9.0). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
- Alternative Name
- MASP2 (MASP2 Products)
- Background
- The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.
- Molecular Weight
- 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result.
- Pathways
- Complement System
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