HRG Protein (AA 19-525) (His tag)

Catalog No. ABIN6239796

This not_set HRG protein is expressed in HEK-293 Cells.

Quick Overview for HRG Protein (AA 19-525) (His tag) (ABIN6239796)

Target: HRG (Histidine-Rich Glycoprotein (HRG))

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 97 %

Product Details

Protein Characteristics: AA 19-525

Purification tag / Conjugate: This HRG protein is labelled with His tag.

Characteristics: Tag location: N-terminal His Tag

Biological Activity Comment: HRG (Histidine-rich glycoprotein) is a plasma glycoprotein which is distinguished by its high content of histidine and proline. HRG binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Thus, a functional binding ELISA assay was constructed to detect the association of rhHRG with heparin. Briefly, Microtiter wells were coated with OVA-conjugated-heparin. rhHRG were diluted serially in 0.01M PBS (pH 7.4). Duplicate samples of 100uL were then transferred to the coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HRG PAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate Solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of rhHRG with heparin was shown in Figure 1 and this effect was in a dose dependent manner The Binding Activity of rhHRG with Heparin.

Application Details

Application Notes: Isoelectric Point: 7

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: HRG (Histidine-Rich Glycoprotein (HRG))

Alternative Name: Histidine Rich Glycoprotein (HRG)

Background: Alternative Names: HPRG, HRGP, Histidine-Proline Rich Glycoprotein, Thrombophilia Due To Elevated HRG

Molecular Weight: 75kDa

UniProt: P04196